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| Ndungu, K; Gitonga, P; Mulinge, M; Kangethe, J; Kibugu, J. K; Munga, L. K; Maina, N; Kagira, J. M; Ngae, G. N; Murilla, G. |
| The efficacy of Triladyl®, a commercial cryomedium for bull semen, in the cryopreservation of both human and animal infective trypanosomes as compared to EDTA Saline Glucose (ESG) 10% glycerol was evaluated in the current study. Cryopreserved Trypanosoma brucei rhodesiense, T. evansi, T. b. brucei and T. congolense were first propagated in irradiated mice. At the peak of parasitemia, parasites were harvested by cardiac puncture and 106,105,104103,102 and 10 dilutions made using whole blood bled from clean mice. These dilutions were divided into two equal portions of 0.5 ml each and cryopreserved in both ESG 10% glycerol and neat Triladly®. The procedure was also repeated with T. congolense and T. vivax species of trypanosomes directly isolated from... |
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Palavras-chave: Trypanosoma; Cryopreservation. |
| Ano: 2009 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2500 |
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| Teixeira,S.M.R.. |
| The study of mechanisms which control gene expression in trypanosomatids has developed at an increasing rate since 1989 when the first successful DNA transfection experiments were reported. Using primarily Trypanosoma brucei as a model, several groups have begun to elucidate the basic control mechanisms and to define the cellular factors involved in mRNA transcription, processing and translation in these parasites. This review focuses on the most recent studies regarding a subset of genes that are expressed differentially during the life cycle of three groups of parasites. In addition to T. brucei, I will address studies on gene regulation in a few species of Leishmania and the results obtained by a much more limited group of laboratories studying gene... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Trypanosoma; Leishmania; Gene expression; RNA; Antigenic variation; VSG. |
| Ano: 1998 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001200001 |
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| Leal,Denise D.M.; O'dwyer,Lucia H.; Ribeiro,Vitor C.; Silva,Reinaldo J.; Ferreira,Vanda L.; Rodrigues,Rozangela B.. |
| Wild animals are exposed to numerous pathogens, including hemoparasites. The Trypanosoma and hemogregarinegroup are frequently reported as parasites in anurans (frogs, tree frogs and toads). The identification of these hemoparasites is usually made through stage observation of their morphology in the peripheral blood of the host. There areno studies, however, based on the biological cycle of these hemoparasites. The objective of the present study was toevaluate the presence of hemogregarines and Trypanosoma spp. in anurans captured in the States of São Paulo andMato Grosso do Sul- Brazil and to perform the morphological and morphometric characterization of these hemoparasites. The species of anurans examined were: Dendropsophus nanus, D. minutus,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Anuran; Hemogregarine; Trypanosoma; Hemoparasites. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652009000200006 |
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| ZHOU, Mo; SUGANUMA, Keisuke; RUTTAYAPORN, Ngasaman; NGUYEN, Thu-Thuy; YAMASAKI, Shino; IGARASHI, Ikuo; KAWAZU, Shin-ichiro; SUZUKI, Yasuhiko; INOUE, Noboru; 五十嵐, 郁男; 井上, 昇. |
| Trypanosoma congolense is a major livestock pathogen in Africa, causing large economic losses with serious effects on animal health. Reliable serodiagnostic tests are therefore urgently needed to control T. congolense infection. In this study, we have identified one T. congolense protein as a new candidate serodiagnostic antigen. The 46.4 kDa protein (TcP46, Gene ID: TcIL3000.0.25950) is expressed 5.36 times higher in metacyclic forms than epimastigote forms. The complete nucleotide sequences of TcP46 contained an open reading frame of 1,218 bp. Southern blot analysis indicated that at least two copies of the TcP46 gene were tandemly-arranged in the T. congolense genome. The recombinant TcP46 (rTcP46) was expressed in Escherichia coli as a glutathione... |
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Palavras-chave: Diagnosis; ELISA; Nagana; TcP46; Trypanosoma. |
| Ano: 2014 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3983 |
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| THEKISOE, Oriel M. M; BAZIE, Raoul S. B; CORONEL-SERVIAN, Andrea M; SUGIMOTO, Chihiro; KAWAZU, Shin-ichiro; INOUE, Noboru; 井上, 昇. |
| This study evaluated the stability of LAMP reagents when stored at 25C and 37C, and also assessed its detection efficiency on different DNA template preparations. Accordingly, LAMP using reagents stored at 25C and 37C amplified DNA of in vitro cultured T. b. brucei (GUTat 3.1) from day 1 to day 15 of reagent storage. There were no significant differences (P>0.05) in detection sensitivity of LAMP among the reagents stored at 25C, 37C and –20C (recommended storage temperature). LAMP using the reagents stored at above-mentioned temperatures amplified serially diluted DNAs (genomic DNA extracted by phenol-chloroform method, FTA card and hemolysed blood) of T. b. gambiense (IL2343) with high sensitivity. Reactions were conducted on the reagents stored... |
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Palavras-chave: Diagnosis; DNA template; LAMP; Parasitic disease; Trypanosoma. |
| Ano: 2009 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2691 |
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| Santalucia,Sérgio; Castro,Jorge Luiz Costa; Raiser,Alceu Gaspar; Castro,Veronica Souza Paiva; Braseiro,Cintia Ramos; Corrêa,Luis Felipe Dutra. |
| Descrevem-se, neste trabalho, as alterações oculares de dois cães naturalmente infectados por Trypanosoma evansi. Os animais apresentaram, ao exame oftalmológico, teste lacrimal de Schirmer I normal, teste de fluoresceína negativo, quemose, hiperemia conjuntival, secreção mucopurulenta, miose, edema de córnea, pressão intraocular diminuída e efeito Tyndall positivo. No esfregaço sanguíneo, foram identificadas formas tripomastigotas, classificadas como pertencentes à espécie T. evansi. Pelos resultados aqui apresentados, concluímos quanto à necessidade de realização de avaliação oftalmológica completa em cães apresentando uveíte, incluindo exames parasitológicos específicos para hemoparasitas. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cão; Efeito Tyndall; Hematozoário; Trypanosoma. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782012001200019 |
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